I use a bunch of random stuff to make sure my microscope is working okay. One of those things is chicken fat... weirdly enough. Coherent Raman imaging is really useful for looking at lipids due to it's naturally high 'nonlinear susceptibility' (jargon for how easy you can move electrons around, and thus change it's local … Continue reading [Images] Random Blob
While attempting to process some of my images from last week, I thought I'd share a cool thing I made (with the help of some others). This is a three-image composite that was taken in a live rat brain using multiphoton microscopy. The red image is NADH autofluorescence, a metabolite responsible for cellular ATP production. … Continue reading [Images] Optical Brain Imaging
These images are from pig mitral valves using Second-Harmonic Generation (SHG) imaging, which highlights collagen fibrils. The mitral valve is responsible for directing blood flow in your heart. Without it, your heart will not be able to pump blood to the rest of your body. Mitral valves are composed largely of collagen and elastin fibrils … Continue reading [Images] Mitral Valve Collagen
This is one of the first biological samples that I got to image with nonlinear Raman imaging. It is a z-stack fly-through of the deeper cortical layers of a fixed brain slice from a rat. The imaging method is Stimulated Raman Scattering (SRS), which uses vibrational contrast that is native to covalent chemical bonds in … Continue reading [Images] Fixed Brain Slice
One of the things we focus on in our lab is applications of label-free observations for research and clinical diagnosis. This image is of a nonmalignant human mammary cell line as part of a breast cancer project. The left image is endogenous autofluorescence , which provides metabolic information about the cells related to electron donor … Continue reading [Images] MCF10A Human Mammary Cells
I took this image of fibroblasts on a culture flask with a FLIR SC8300 high-resolution thermal camera. Thermal imaging is not something typically used on cells, largely because it's diffraction limited resolution is on the order of ~3 microns (the size of most mammalian cells). We had a unique opportunity to try this in our … Continue reading [Images] 3T3 Fibroblasts
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