I use a bunch of random stuff to make sure my microscope is working okay. One of those things is chicken fat… weirdly enough. Coherent Raman imaging is really useful for looking at lipids due to it’s naturally high ‘nonlinear susceptibility’ (jargon for how easy you can move electrons around, and thus change it’s local charge dynamics). In this picture, I’m using CARS (coherent anti-Stoke Raman scattering, green) and SRS (stimulated Raman scattering, red) to look at a lipid-dominant Raman signal (2850cm^-1). In most cases, you expect CARS and SRS signal to line up pretty well. In this case, that did not happen… and I am not exactly sure why. But nonetheless it resulted in a cool looking image.
[Images] Random Blob
Published by Wilson Adams
Graduate Student working on my Ph.D. in Biomedical Engineering. Primary research interests are applications of nonlinear imaging to answer questions for neuroscience and other biomedical applications. Avid musician, hiker, homebrewer, personal finance junkie, foreign affairs and international relations enthusiast. View all posts by Wilson Adams