This is one of the first biological samples that I got to image with nonlinear Raman imaging. It is a z-stack fly-through of the deeper cortical layers of a fixed brain slice from a rat. The imaging method is Stimulated Raman Scattering (SRS), which uses vibrational contrast that is native to covalent chemical bonds in samples. This particular series of SRS images is tuned to highlight lipid-dominant signal, which comprises much a particular cells organelles and membranes.